At Exonova Lifesciences, we employ a range of advanced technologies to isolate and purify and characterize Extracellular Vesicles for further diagnostics and therapeutic applications.

Chromatography & Filtration methods for Exosome Isolation & Purification

• Size-Exclusion Chromatography (SEC): A precise method to isolate exosomes while preserving their biological activity, using an automated platform for consistent, high-quality fraction collection.
• Ion-Exchange Chromatography: Advanced separation methods to achieve ultra-pure exosome outputs.

Membrane Filtration:

• TFF (Tangential Flow Filtration): Concentrating and purifying exosomes without compromising their integrity.
• Sterile Filtration: Ensuring contamination-free final products for clinical applications.

Extracellular Vesicle Characterization
Particle Analysis: Cutting-edge techniques for determining accurate particle size, concentration, and surface charge.
Surface Marker Identification: Western blotting to identify and validate exosome specific protein markers.
RNA Analysis: PCR techniques for identifying and validating RNA markers specific to exosomes.

Bioprocessing & Scale-Up
Bioreactors: Scalable systems optimized for high-yield exosome production.
Automation: Advanced automated platforms to ensure consistent quality and reproducibility during large-scale production.